GsMTx-4 peptide (Atto 647N)
Reactivity: Grammostola rosea
Host: Synthetic
BP, FACS, FM, IF, LCI
Catalog No. ABIN7582206
Quick Overview for GsMTx-4 peptide (Atto 647N) (ABIN7582206)
Target
GsMTx-4
Origin
Grammostola rosea
Source
Synthetic
Application
Blocking Peptide (BP), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)
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Peptide Type
- Synthetic
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Purification tag / Conjugate
- Atto 647N
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Sequence
- GCLEFWWKCN PNDDKCCRPK LKCSKLFKLC NFSF-NH2
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Specificity
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Toxin target: NaV1.7 channels, TRPC1, TRPC6, Piezo1, Phe34 - C-terminal amidation ATTO Fluor-647N
Disulfide bonds between: Cys2-Cys17, Cys9-Cys23, and Cys16-Cys30 -
Characteristics
- GsMTx-4-ATTO Fluor-647N (ABIN7582206) is a highly pure, natural, and biologically active conjugated peptide toxin. Benefits of GsMTx-4-ATTO Fluor-647N: Localization and distribution Clustering and internalization kinetics Live cell imaging Single cell detection Binding kinetics Direct flow cytometry We gladly take on collaboration projects. Please Contact Us.
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GsMTx-4 peptide (Biotin)
Reactivity: Grammostola rosea Host: Synthetic BP, FACS, FM, IF, LCI
GsMTx-4 peptideReactivity: Grammostola rosea Host: Synthetic BP
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Application Notes
- Antigen Preadsorption Control: 0.1 μM - 5 μM
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 mL conical vial. The product is soluble in pure water to high-micromolar concentrations (5 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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Storage
- -20 °C
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Storage Comment
- Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.,Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.
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- GsMTx-4
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Background
- M-TRTx-Gr1a, M-theraphotoxin-Gr1a, GsMTx4,GsMTx-4 is a 34 amino acid peptidyl toxin originally isolated from the Grammostola rosea (Chilean rose) tarantula venom and belongs to the huwentoxin-1 family1. This toxin inhibits different channels and in addition has antimicrobial activity. It blocks cation-selective mechanosensitive ion channels (strech-activated channels, SACs), without having an effect on whole-cell voltage-sensitive currents1. In addition, it inhibits atrial fibrillation2 as well as the membrane motor of outer hair cells3 at low doses. A medium toxicity on a large spectra of voltage-gated Na+ channels, namely NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.5, NaV1.6 and NaV1.7 was reported. GsMTx-4 also inhibits K+ channels KV11.1 and KV11.2, whereas it does not inhibit K+ channels KV1.1 (IC50 > 85 μM), KV1.4 (IC50 > 85 μM) and KV11.3 (IC50 = 53 μM)4. GsMTx-4 was also found to inhibit both TRPC1 and TRPC6 channels5,6, as well as Piezo1, the mechanosensitive channel7. Antimicrobial activity is shown against several Gram-positive and Gram-negative bacteria as well8.
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Molecular Weight
- 4723 Da
Target
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